A dissertation which taught me that we have to look over our enzymes

PCR is like cars. The car was invented a long time ago, but it remains important, and it is constantly developed and refined. Today I had the pleasure of examining Maja Sidstedt (as a member of the committee, Titia Sijen was the faculty opponent, and an excellent job she did). And the thesis was focused on PCR, actually on substances inhibiting PCR and how polymerases reacts to inhibiters. It was a good thesis and on something I found interesting.

Over the years we (in Stockholm) have happily PCRed away with AmpliTaq gold. Thus it is a pity that that particular enzyme ended up on the bottom of Majas list of which enzymes preforms best in the presence of humic and fulvic acids. Way bellow for example Immolase. There was much to be learned about PCR inhibitors, Taq quality, emulsion PCR, digital PCR and much more today. It was a good day. And for you who are interested in these kinds of things, Maja has published most of it, and perhaps Organic chemistry in Lund sells her thesis.

Peter Rådström, Johannes Hedman (supervisors), Anders Götherström, Lisa Wasserström (committee members), Maja Sidstedt (respondent), Titia Sijen (faculty opponent), and Claus Børsting (committee member).